DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND VALSARTAN ITS STANDARD MIXTURE USING BOXBEHNKEN DESIGN

Abstract

High performance thin layer chromatography (HPTLC) method has been developed for the separation of cilnidipine and valsartan using pre-coated silica gel aluminium plate 60F254, with UV detection at 300 nm. Box- Behnken design was applied for multivariate optimization of the experimental conditions of HPTLC method. Three independent factors: Ethyl acetate content in mobile phase composition, saturation time and migration distance whereas Rf was taken as response which was used to design mathematical models. The predicted optimum assay conditions consisted of toluene: methanol: ethyl acetate: GAA (6:2:2:0.1, v/v/v/v), respectively as the mobile phase. The method was validated according to ICH guidelines.