EVALUATION OF THE ANTIOXIDANT PROPERTY OF THE ETHANOLIC EXTRACTS OF CHONEMORPHA MACROPHYLLA ROOTS

Abstract

Ethanol extracts of C. macrophylla roots were prepared using 50% and 95% ethanol and were named as CMRH and CMRE respectively. Preliminary phytochemical studies were performed and identified the presence of polyphenols and flavonoids. Antioxidant studies of both the extracts were performed using ABTS scavenging assay, DPPH scavenging assay, nitric oxide scavenging assay and lipid peroxidation assay methods. The IC50 values of CMRH, CMRE and rutin in ABTS assay were 5.63±0.74, 2.30±0.20 and 3.03±0.06 μg/ml respectively. In the DPPH assay, the IC50 values were 71±0.58, 72.33±2.89 and 32.08±0.88 μg/ml respectively. IC50 values obtained in nitric oxide scavenging assay were around 1 mg/ml for both the samples. The IC50 values obtained in LPO assay were 210±10.0 μg/ml and 230±2.0 μg/ml for CMRE and BHA respectively. The IC50 value obtained in LPO assay for CMRH was above 1 mg/ml. All of the above results indicate the antioxidant potential of both the extracts and it can possibly due to the polyphenols and flavonoid antioxidants present in the plant.