QUANTIFICATION OF 2, 4-DIAMINOPYRIMIDINE 3- OXIDE IN MARKETED HAIR GROWTH FORMULATION USING RP-HPLC

Abstract

2,4-diaminopyrimidine 3-oxide is widely used as hair growth promoter agent in cosmetics and pharmaceutical preparations. A simple RP-HPLC method was developed for quantification of 2,4-diaminopyrimidine 3-oxide in marketed hair growth formulation. The method was developed using C18 column and mobile phase consisted of purified water: methanol (75:25) with flow rate of 0.8 mL/min. The eluent was monitored at 225 nm. The lineraity of the standard 2,4-diaminopyrimidine 3-oxide was performed between concentration 0.01 to 0.1 mg/mL and LOD and LOQ of the method were found. The method was found to be linear. The developed method was successfully applied for quantification of 2,4-diaminopyrimidine 3-oxide in marketed formulation.